GITR/NFκB-Luc/Jurkat
CBP74012
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| I. Background | |
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糖皮质激素诱导的肿瘤坏死因子受体相关蛋白（GITR，也称为 TNFRSF18 或 CD357），属于肿瘤坏死因子受体超家族（TNFRSF），它在多种免疫细胞上表达，包括 效应 T 细胞和调节性 T 细胞。GITR 在调节免疫系统中发挥重要作用，尤其是在 T 细胞的 活化和调节性 T 细胞（Treg）的功能中。 |
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| II. Description | |
GITR NFκB-Luc Jurkat 报告基因药靶模型很好的模拟了体内 GITR 的信号转导过程， 原理见下图所示。 Figure 1. GITR NFκB-Luc Jurkat 细胞模型原理图
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: |
GITR-NFκB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+400 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 2. Recombinant Jurkat stably expressing human GITR.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human TNFSF18 in GITR-NFκB-Luc Jurkat(C1) Dose Response of GITR NFκB-Luc Jurkat(C1) Stimulated by GITRL CHO (C1).
