CD40/NFκB-Luc/HEK293

CBP74009

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索取Protocol

产品描述

产品数据库

I. Background
CD40 是肿瘤坏死因子受体超家族（TNFRSF）成员，作为免疫调节的关键分子，广泛参与 B 细胞活化、抗原呈递细胞（APC）成熟及 T 细胞免疫应答。其配体 CD40L（CD154）主要表达于活化的 T 细胞，通过 CD40-CD40L 互作桥接先天性与适应性免疫，是肿瘤免疫治疗、自身免疫病及感染性疾病的热门靶点。 CD40 信号转导主要利用 TNF 受体相关因子的衔接蛋白，导致经典和非经典 NFκB 通路、MAPK、PI3K 和 PLCγ的激活。它们的激活导致特征性下游效应，包括转录激活、细胞骨架重排和细胞存活。

II. Description
CD40 NFκB-Luc HEK293 报告基因药靶模型很好的模拟了体内 CD40 的信号转导过程，原理见下图所示。 Figure 1. CD40 NFκB-Luc HEK293 细胞模型原理图

III. Introduction
Host Cell:	HEK293
Expressed gene:	CD40
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-κB cell line, NFκB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	MEM+10%FBS+1% NEAA+1 mM NaP+1 μg/ml Puromycin+100 μg/ml Hygromycin B
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Human
Tissue:	kidney
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 2. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40.
Figure 3. Dose Response of Human CD40L in CD40 NFκB-Luc HEK293( C4). Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Blocking of hCD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293(C4). Blocking of CD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293 Cell(C4) with CD40L Target Cell(C7).