MCF7-Luc
CBP30105L
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| I. Introduction | |
| Host Cell: | MCF7 ER positive |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+ 10 µg/ml human insulin+ 1μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Mammary gland breast;derived from metastaticsite:pleural effusion |
| Disease: | Adenocarcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.. |
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| IV. Stablity Test | |
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Figure. Stablity test results. MCF7-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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